Jonathan Fascetti
fascetti.j@wustl.edu
Cell: 781-521-2015
I took a lab-intensive course in thermodynamics with an emphasis on experimental design and presentation.
During the second half of the semester, my partner and I designed a prospective lab for the course. Our
experiments used solution calorimetry to predict the activity of various digestive enzymes using an
over-the-counter meal supplement powder. Throughout the experimental design process, we iterated our
protocols based off current literature and feasibility for a class-scaled laboratory.
Below is the poster we made which showcases the specifics of the lab we created as well as our results.
During this 10-week summer internship program, I worked in a product-oriented research lab at New England
Biolabs with a focus on nucleic acid amplification techniques, specifically LAMP. My specific project had
two sides: to investigate the effects of reaction volume on detection tendencies for genomic targets and to
optimize workflows for repetitive protocols on automated liquid handling platforms. I gained hands on
experience with more lab equipment, frequently designed experiments, and programmed various liquid handler
platforms. In addition to these technical experiences, an invaluable take away from the summer was my
appreciation for the importance of communication within a team. I frequently prepared concise presentations
to deliver to the lab group regarding the progress of the project and areas I sought advice. Between putting
together these communications and hearing colleagues delivering their own, I came to appreciate that the
collaboration directly gave rise to progress to all our projects.
The poster below is of the poster I created summarizing my project which I presented during a company-wide
symposium at the end of the summer.
During the summer before starting at Washington University in St. Louis, I worked on a project with a peer I
graduated high school with and a mentor to create new software in the realm of image processing. We were
very thorough in how we organized our research, code, and progress. Throughout the summer, I didn’t just
learn a lot about image processing (e.g., feature detection, homography, and warping), but I also gained
valuable experience with regard to project management. We practiced Agile development methods (had frequent
standup meetings and periodic spring review sessions), organized our code in GitHub (frequently committing
and pulling updates to make sure we did not lose any code between the three of us), and worked together to
problem solve through issues while considering multiple variables such as run time, usability, etc. To make
our software usable within an application, more time than a summer is required, so for the time being the
project is on hold until all our schedules allow us to commit time to it.
The image below is an example of how I used homography methods to warp a test image (middle) to the
perspective of a reference image (left) by means of matched-feature locations (black and white dots on test
and refrence image) The resultant image is on the right side.
At the end of my senior year of high school, my school implemented the use of ‘antibacterial’ grips and stickers that were placed on door handles and other common-touch places with hopes of mitigating the spread of germs during in-person school mid-pandemic. I was curious whether the grips were truly antibacterial, so, through an independent study, I put them to the test. Throughout the term, I meticulously crafted a series of experiments, performed them, and went through the engineering design process by redesigning my procedures. I learned a lot with regard to basic lab skills like mixing and pouring ager plates, swabbing methods, and how to execute procedures while maintaining a sterile environment. My experiments were unfortunately cut short due to the end of the school year but if I had more time I would have sent out bacterial samples I had collected to be sequenced and taxonomically identified so I could do further analyze my results and predict what types of bacteria the grips may be effective against. The biggest take away I had from the project, though, was how much planning is required to make a well-rounded experiment that accounts for many different types of uncertainty.
As part of the course Introduction to Biomedical Engineering, I worked with a small group of peers to practice needs finding by discovering a ‘need’ in a specific population, and design a solution for them. Our group explored a kidney disease patient's perspective and designed and proposed a solution for a way to perform effective interim dialysis during their recovery from invasive long term preparatory surgeries. We proposed that electric stimulation can be directed toward veins which could increase blood flow sufficiently to perform dialysis. We used current research to defend our solution as well as mechanical principles we learned through the course like Poiseuille's law. Throughout the project we had to iterate and engineer various possible solutions to arrive at one that we all were happy with. I not only learned a lot about preparatory methods for dialysis but also how to work with a group of peers to generate ideas and organize a poster in an effective way.
In various classes, clubs, and out of personal interest, I have pursued learn how to use computer aided design (CAD) software. The example below is from an engineering class I took senior year of high school and was mainly focused around translating physical measurements into CAD models. I’ve also gained some experience with CAD through a robotics team I was involved with in high school where I helped design parts (e.g., sliding clamps) that I made with a 3D printer to go on our autonomous water vehicle. In instances like this, I learned a lot about the practicality aspects of using CAD to make objects through means of 3D printing like how to deal with inevitable errors. I have also been able to continue to exercise my CAD skills through courses in college.
As part of my senior year final project, I pursued an internship with a Boston University linguistics faculty member where we developed a way to visualize her data from user input. In short, we synthesized a method to match user input to known data stored in CSV (comma separated value) files and display the output data in a web interface. This professor uses this tool we developed together as a data visualization method for her research group. While this project allowed me to take away specific skills (like moving data around different platforms, datatypes, and programming languages), I also developed strengths that are applicable more generally as well. For example, I learned how to view initial ideas from different perspectives in order to create a more effective solution, apply and progress my prior programming knowledge to new circumstances, and I also had some exposure to fascinating linguistic interpretation methods.
Starting in my sophomore year of high school I participated in a club at my school called BeaverAUV. In the
club, we design, program, and build an autonomous underwater vehicle (AUV) to compete in an international
collegiate competition called RoboSub. When I was part of the club, I mainly worked on the computer vison
aspects of the AUV. In short, the largest endeavor I took on was developing a way for our AUV to detect
gates, which it would have to independently navigate through in the competition. Starting as someone who had
never done anything related to image processing, I had to do a lot of research on which methods would fit
our needs, and how to translate them over to the AUV itself. I also had the opportunity to collaborate with
other team members and learn what they were working on too, like piece design with CAD, motor control, and
how to generally manage a club and funding.
The image below is an example of what an output from my gate detection program was, which would later be
implemented to the AUV for live use.
As an independent study in the beginning of my senior year of high school, I investigated microbiomes,
techniques to analyze them, and their potential applications. In this study, I extensively researched the
relevant scientific methods and biological systems of microbiomes and their implication onto human health. I
then synthesized my research and developed a website designed to teach people about the importance of
microbiomes and the potential impact they have on human medicine. Throughout the project, I really enjoyed
having the freedom to seemingly endlessly dig deeper and deeper into a field of science, especially since a
lot of the literature I was reading was very new. I then was faced with taking all of the information I had
collected and translating it into a readable form for someone who was not as familiar with the field as I
am.
Check out the site I made here.
Jonathan Fascetti's Portfolio